Funded projects

Architecture of membrane associated machineries at sub-nanometer resolution

2015 - D. Levy (UMR168), A. Bertin (team Levy UMR168), P. Bassereau (UMR168), S. Mangenot (team Mangenot UMR168), C. Delevoye (team Raposo UMR144)

Build 3D models of proteins at sub-nanometer resolutions understand how these proteins interacts, form specific assemblies and remodel membranes at the molecular level and integrate these 3D models in the mechanisms deduced from approaches of cell biology and physics of membranes. Implement within the PICT, 3D reconstruction of proteins from high resolution electron microscopy images for a broader audience at Curie.

1. Architecture of Biogenesis of Lysosome-related Organelles Complex-1 (BLOC-1)

2. Septins assembly on membranes and formation of membrane diffusion barrier

3. Characterization of Ezrin membrane tethering

4. Multiscales analysis of membrane fission by ESCRT-III proteins complex

Methods/Technologies:

  • Titan Krios electron microscopy
  • GUVs with confocal microscopy
  • Optical tweezers
  • In vitro system of mixed synthetic lipid vesicles

Keywords: cryoEM, biomimetics, membrane dynamics, fission