Tran / Anne Paoletti

Cytoskeleton Architecture and Cellular Morphogenesis

Phong Tran / Anne Paoletti
Scientific keywords: cytokinesis, cytoskeleton, division, Live cell imaging, Mammalian cells, Microfluidics, mitosis, morphogenesis, Yeast genetics
Technics Used in the Lab: live cell imaging, microfluidics, yeast genetics, mammalian cells

Our team, co­directed by Phong Tran and Anne Paoletti, explores how the cytoskeleton is organized, how it controls the establishment of functional membrane domains devoted to polarized cell growth or cell division, and how it is remodeled at mitotic entry for the assembly of the mitotic spindle and contractile ring, two complex molecular machines promoting chromosome segregation and cytokinesis. Most our studies are performed in the fission yeast model system, where cell organization is stereotyped and the cytoskeleton relatively simple (Fig. 1). We combine classical molecular genetics with state­of­the­art live cell microscopy approaches in combination with micro­fabricated devices to control cellular environment. More recently, we have started exploring evolutionary conserved pathways in mammalian cells.

Schematic of fission yeast actin and microtubule cytoskeleton architecture throughout the cell cycle.

Schematic of fission yeast actin and microtubule cytoskeleton architecture throughout the cell cycle.