Proper division plane positioning is essential to achieve faithful DNA segregation and to control daughter cell size, positioning, or fate within tissues. In Schizosaccharomyces pombe, division plane positioning is controlled positively by export of the division plane positioning factor Mid1/anillin from the nucleus and negatively by the Pom1/DYRK (dual-specificity tyrosine-regulated kinase) gradients emanating from cell tips. Pom1 restricts to the cell middle cortical cytokinetic ring precursor nodes organized by the SAD-like kinase Cdr2 and Mid1/anillin through an unknown mechanism. In this study, we show that Pom1 modulates Cdr2 association with membranes by phosphorylation of a basic region cooperating with the lipid-binding KA-1 domain. Pom1 also inhibits Cdr2 interaction with Mid1, reducing its clustering ability, possibly by down-regulation of Cdr2 kinase activity. We propose that the dual regulation exerted by Pom1 on Cdr2 prevents Cdr2 assembly into stable nodes in the cell tip region where Pom1 concentration is high, which ensures proper positioning of cytokinetic ring precursors at the cell geometrical center and robust and accurate division plane positioning.
Valorization Manager position for Labex DEEP and Labex Celtisphybio programs, Institut Curie, Paris
The Institut Curie (http://www.curie.fr) is a world-class multidisciplinary cancer research center combining research in biology, genetics, soft matter physics, organic and medicinal chemistry. The activities of the Labex valorization manager will cover both DEEP and CelTisPhyBio, two Labex programs of the Institut Curie.
Jaume Casademunt is a professor of physics at the University of Barcelona. His research background includes expertise in non-linear and non-equilibrium statistical physics, in particular in fluctuation phenomena, pattern formation, and fluid dynamics. In the last decade his research been directed towards the application of the concepts and tools of the above disciplines to biological problems, with special attention to collective, self-organized phenomena. Recent research contributions include the areas of: (i) Mechanics of epithelial tissues; (ii) Molecular motors and intracellular traffic; (iii) Membrane-cortex adhesion and bleb formation; (iv) Actin-based cell motility; and (v) Dynamics of cultured neuronal networks. His most recent interests are focused on modeling and understanding collective cell migration and tissue mechanics in epithelia from a hydrodynamic point of view. From a more fundamental point of view, he is also interested in understanding the nature of instabilities and turbulence in active media.