Perez

Dynamics of Intracelllular Organization

Franck Perez
Scientific keywords: Golgi apparatus, Intra-cellular traffic, Microtubules, Recombinant Antibodies, Secretion
Technics Used in the Lab: Real time imaging, immunofluorescence, High content screening, recombinant antibody selection, molecular biology

We study two aspects of cellular dynamics: the Golgi apparatus and microtubule dynamics.

The Golgi apparatus – We analyzed its dynamics and maintenance. We have now developed a generic transport assay, the RUSH assay (Fig 1), that enables to study of a large diversity of cargo in living cells. It is also usable for automated High Content Screening.

Microtubule dynamics – We studied the role proteins like CLIP-170 in the dynamic instability of microtubules. We recently selected an antibody sensitive to tubulin conformational changes. This allowed us to verify the presence of a GTP cap and to propose a new model of microtubule dynamic instability based on the discovery of GTP tubulin islands (Fig. 2).

We implemented a variety of methods such quantitative analysis of intracellular trafficking or microtubule dynamics. We are also involved in the development of novel approaches and tools (e.g. High Content Screening, recombinant antibodies or RUSH). We recently constructed an library of recombinant antibodies that we use both for fundamental question liked to cell biology and to therapeutic questions related to the diagnosis ad treatment on cancers.

Figure 1 : The RUSH assay. Description of the system (left) and its application for ER-Golgi transport (right)

Figure 1 :
The RUSH assay. Description of the system (left) and its application for ER-Golgi transport (right)

 

Figure 2 A GTP island-based model for microtubule dynamic instability

Figure 2
A GTP island-based model for microtubule dynamic instability