Ludger

Traffic, Signaling and Intracellular Delivery

Johannes Ludger
Scientific keywords: drug delivery, endocytosis, immunotherapy, interferon receptors and Jak/Stat signaling, intracellular delivery, protein toxins, retrograde transport, vector technology
Technics Used in the Lab: Clathrin-independent endocytosis, intracellular/retrograde trafficking, Cellular biochemistry: quantitative trafficking assay, trafficking probes and tracers, Model membrane reconstitution of trafficking events, Glycosphingolipid synthesis and reconstitution in cells, Proteomics of clathrin-independent endocytosis and retrograde transport, Membrane mechanics, biology/physics interface

The two teams of the Traffic, Signaling and Delivery group are focused on several aspects of how cells communicate with their environment through endocytosis (the process whereby cells internalize proteins, solutes etc. from the extracellular space). We are also investigating how endocytic pathways can be exploited to deliver therapeutic and diagnostic compounds into cells. We study novel trafficking pathways and mechanisms, especially that of the bacterial Shiga toxin and of the interferon receptors, which we also use to address the relationship between traffic and signaling.

Figure 1: Retrograde intracellular trafficking (Johannes and Popoff, 2008, Cell 135: 1175-1187).

Figure 1: Retrograde intracellular trafficking (Johannes and Popoff, 2008, Cell 135: 1175-1187).

Figure 2: Membrane sorting and Jak/Stat signaling of interferon receptors.

Figure 2: Membrane sorting and Jak/Stat signaling of interferon
receptors.